Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.25 µg)/test
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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 660, 700
• APC
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 650
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It synergizes with TNFα to inhibit cell proliferation and modulate immune responses.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometry of stimulated human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in 100 µL final volume. Cell count: 10⁵–10⁸ cells/test (empirically determined).

Fluorophore Information:
APC-eFluor 780 emits at 780 nm, excited by a 633 nm red laser. Ensure instrument compatibility.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness or compensation.

Excitation/Emission:
633–647 nm / 780 nm (Red Laser)

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation and immune interferon produced mainly by T-lymphocytes and NK cells in response to antigens and cytokines.

• Dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits
• Functions: antiviral, antiproliferative, MHC induction, macrophage activation, B-cell immunoglobulin enhancement
• Species specificity: human IFNγ does not cross-react with mouse
• Regulated by IL2, FGF basic, EGF (upregulated); inhibited by vitamin D3 or DMN
• Mutations associated with aplastic anemia

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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