Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), APC-eFluor 780, eBioscience
인간 IFN-γ를 인식하는 4S.B3 단클론 항체로, APC-eFluor 780 형광체로 결합되어 유세포분석(Flow Cytometry)에 최적화됨. 5 µL/test로 사용하며, 광민감성이 있어 암실 보관 필요. 연구용으로 세포 내 염색 및 사이토카인 분석에 적합.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), APC-eFluor 780, eBioscience
Applications
- Flow Cytometry (Flow)
Tested dilution: 5 µL (0.25 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4S.B3 |
| Conjugate | APC-eFluor™ 780 |
| Excitation/Emission Max | 756/785 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853010 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 660, 700
- APC
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 650
- eFluor 450, 506, 660
- FITC, PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It synergizes with TNFα to inhibit cell proliferation and modulate immune responses.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometry of stimulated human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in 100 µL final volume. Cell count: 10⁵–10⁸ cells/test (empirically determined).
Fluorophore Information:
APC-eFluor 780 emits at 780 nm, excited by a 633 nm red laser. Ensure instrument compatibility.
Light Sensitivity:
Protect from light to prevent photo-induced oxidation.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness or compensation.
Excitation/Emission:
633–647 nm / 780 nm (Red Laser)
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation and immune interferon produced mainly by T-lymphocytes and NK cells in response to antigens and cytokines.
- Dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits
- Functions: antiviral, antiproliferative, MHC induction, macrophage activation, B-cell immunoglobulin enhancement
- Species specificity: human IFNγ does not cross-react with mouse
- Regulated by IL2, FGF basic, EGF (upregulated); inhibited by vitamin D3 or DMN
- Mutations associated with aplastic anemia
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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