Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), APC-eFluor 780, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 26 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 563 / 737 / 805
• Brilliant Violet 605 / 786
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio64DEC17 antibody reacts with human IL-17A and is a neutralizing antibody.
Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Human IL-17A exists as glycosylated 20–30 kD homodimers and enhances expression of ICAM-1 in fibroblasts. It stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2, and supports CD34+ progenitor proliferation and neutrophil maturation.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) represent a distinct subset from Th1 and Th2 cells. IFN-γ and IL-4 negatively regulate Th-17 cell generation.

Intracellular staining by eBio64DEC17 identifies the same population as eBio64CAP17.
Pre-titrated for intracellular flow cytometric analysis at 5 µL (0.125 µg) per test (final volume 100 µL).
Cell number: 10⁵–10⁸ cells/test (empirically determined).

Fluorochrome Information:

• Excitation: 633–647 nm
• Emission: 780 nm
• Laser: Red
• Light sensitivity: Protect from light (photo-induced oxidation risk)
• Filtration: 0.2 µm post-manufacturing filtered

Fixation Guidance:
Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.
Clone-specific performance should be determined empirically.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases.
IL-17A is a 32 kDa disulfide-linked homodimer (136 amino acids), signaling through the IL-17 receptor (IL-17R/CDw217).
It regulates NF-κB and MAPK activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide production.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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