Thermo Fisher Scientific NFIA/NF1A (Nuclear Factor 1A) (Transcription Factor) Monoclonal Antibody (PCRP-NFIA-2C6)
NFIA/NF1A 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Immunoprecipitation (IP) | 1–2 µg |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-NFIA-2C6 |
| Immunogen | Recombinant fragment of human NFIA protein corresponding to protein domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich, carboxy terminal transactivation domain. The NF-1 protein recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter region of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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