Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), eFluor 450, eBioscience
마우스 IgM의 μ 중쇄를 인식하는 단클론 항체로, eFluor 450 형광체로 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 405 nm에서 여기되어 445 nm에서 방출됩니다. B세포 발현 연구 및 면역분석용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 25 publications |
| ELISA | - | View 5 publications |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2574072 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 570
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other immunoglobulin classes such as IgD, IgG, or IgA. IgM is expressed intracellularly during early B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral tissues. Fluorochrome-conjugated II/41 can be used as a detection secondary for mouse IgM.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended usage ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Fluorochrome Information:
eFluor™ 450 is an alternative to Pacific Blue™, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with violet laser detection.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during an immune response and is the largest immunoglobulin in serum. Composed of five heavy and five light chains, it participates in complement activation and antigen agglutination. It plays a crucial role in innate immunity and is widely used in ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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