Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor 450, eBioscience
마우스 TCR V alpha 2 체인을 인식하는 B20.1 단클론 항체로, eFluor 450 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출됩니다. 4°C에서 암소 보관, 동결 금지.
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- 48-5812-8x (2개 옵션)
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- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor 450, eBioscience
Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 6 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B20.1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_10804399 |
Available Formats
- APC
- APC-eFluor 780
- Biotin
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Red 700
- NovaFluor Yellow 570
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. It recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes. This antibody also reacts with the products of T cell receptor V delta 8 due to high homology.
Applications Reported: Flow cytometric analysis.
Applications Tested: ≤ 0.125 µg per test (in 100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
eFluor™ 450 is an alternative to Pacific Blue™. It emits at 445 nm and is excited with the violet laser (405 nm). Ensure your instrument can detect this fluorochrome.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered
Target Information
T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response. Recognition of self-peptides is linked to autoimmune disease, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis and lupus patients, potentially modulating inflammatory responses. Most human T cells express TCR alpha-beta with CD4 or CD8; a small subset lacks both (double negative). Increased alpha-beta DN T cells have been observed in autoimmune and immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelium, exhibit limited diversity and distinct antigen recognition, with subsets showing antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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