Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor 450, eBioscience

Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• Biotin
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. It recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes. This antibody also reacts with the products of T cell receptor V delta 8 due to high homology.

Applications Reported: Flow cytometric analysis.
Applications Tested: ≤ 0.125 µg per test (in 100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.

eFluor™ 450 is an alternative to Pacific Blue™. It emits at 445 nm and is excited with the violet laser (405 nm). Ensure your instrument can detect this fluorochrome.

Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response. Recognition of self-peptides is linked to autoimmune disease, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis and lupus patients, potentially modulating inflammatory responses. Most human T cells express TCR alpha-beta with CD4 or CD8; a small subset lacks both (double negative). Increased alpha-beta DN T cells have been observed in autoimmune and immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelium, exhibit limited diversity and distinct antigen recognition, with subsets showing antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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