Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 450, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent from resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cell proliferation.

SolA15 also reacts with human, non-human primate, and canine Ki-67.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Recommended ≤ 0.125 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with violet laser detection.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin structures and shows distinct localization patterns across cell cycle phases. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases through mitosis.

In neoplastic tissues, Ki-67 index correlates with tumor grade and prognosis, providing valuable information on cell growth fraction. It is also used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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