Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 450, eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 마커 분석에 적합합니다. eFluor 450 형광체 결합으로 405 nm에서 여기되어 445 nm에서 방출됩니다. Flow cytometry 및 ICC/IF에 활용되며, 인간, 마우스, 랫트 등 다양한 종에 반응합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 33 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151155 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent from resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cell proliferation.
SolA15 also reacts with human, non-human primate, and canine Ki-67.
Applications Reported:
Intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Recommended ≤ 0.125 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with violet laser detection.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin structures and shows distinct localization patterns across cell cycle phases. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases through mitosis.
In neoplastic tissues, Ki-67 index correlates with tumor grade and prognosis, providing valuable information on cell growth fraction. It is also used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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