Thermo Fisher Scientific P-Glycoprotein Monoclonal Antibody (UIC2)
인간 및 비인간 영장류 반응성을 가진 P-Glycoprotein 단클론 항체(UIC2). 다양한 면역학적 응용(IHC, ICC, Flow, IP)에 사용 가능. 단백질 A 정제, 1 mg/mL 농도, 비결합형 액상 형태. 장기 보관 시 -20°C에서 냉동 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UIC2 |
| Immunogen | Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538434 |
Product Specific Information
- This antibody does not react with mouse or rat.
- Heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) is recommended for paraffin sections.
- Suggested positive control for IHC: human kidney.
- For FACS analysis, use 10 µL of working dilution to label 1×10⁶ cells in 100 µL.
- Mouse anti-Human CD243, clone UIC2 recognizes an extracellular conformational epitope of CD243 (MDR1/Pgp), a multi-pass transmembrane protein and member of the ABC transporter family.
Target Information
The membrane-associated protein encoded by this gene is part of the ATP-binding cassette (ABC) transporter superfamily.
ABC proteins transport various molecules across cellular membranes and are divided into seven subfamilies: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White.
This protein belongs to the MDR/TAP subfamily, which is involved in multidrug resistance.
It acts as an ATP-dependent drug efflux pump with broad substrate specificity, reducing drug accumulation in resistant cells and mediating resistance to anticancer drugs.
Additionally, it functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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