Thermo Fisher Scientific CD204 Monoclonal Antibody (2F8)
CD204 단백질을 인식하는 쥐 단클론 항체(클론 2F8)로 다양한 종(Fish, Mouse, Pig)에 반응합니다. Western blot, IHC(F), Flow cytometry, ELISA, IP 등에 사용 가능하며, 비결합형 형태로 제공됩니다. 단백질 G로 정제되었으며 1 mg/mL 농도의 액상 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fish, Mouse, Pig |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F8 |
| Immunogen | Raw 264 cell line (Target: CD204, UniProt ID: P30204-1, Antigen range: 1–458 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538003 |
Additional Formats
Product Specific Information
- In Western blot, this antibody recognizes CD204 in J774 cells under non-reducing conditions only.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
CD204 gene encodes the class A macrophage scavenger receptors (types 1, 2, and 3), generated by alternative splicing. These trimeric membrane glycoproteins are macrophage-specific and play roles in processes such as atherosclerosis, Alzheimer’s disease, and host defense.
Type 1 and 2 isoforms mediate endocytosis of modified LDLs, while type 3 lacks this function due to altered intracellular processing and can inhibit the activity of the other isoforms, suggesting a regulatory mechanism for scavenger receptor activity.
Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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