
Thermo Fisher Scientific Dectin 1 (soluble) Monoclonal Antibody (2A11)
마우스 Dectin-1을 인식하는 Rat monoclonal antibody (Clone 2A11)로, IHC(F), Flow Cytometry, IP 등 다양한 응용에 적합합니다. 비결합형(Unconjugated) 형태이며, PBS 버퍼에 1 mg/mL 농도로 제공됩니다. H2O2 및 포름알데히드 처리에 민감하므로 아세톤 고정 권장.
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Thermo Fisher Scientific Dectin 1 (soluble) Monoclonal Antibody (2A11)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | 1:10–1:50 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A11 |
| Immunogen | Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537994 |
Additional Formats
Product Specific Information
- Epitope recognized by 2A11 is sensitive to H₂O₂; quenching with 0.3% H₂O₂ should be performed after incubation with the primary antibody.
- The epitope is also sensitive to formaldehyde fixation and tissue processing; acetone fixation is recommended for frozen sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Rat anti-Mouse Dectin-1 antibody, clone 2A11, recognizes the murine beta-glucan receptor (Dectin-1).
Target Information
CLEC7A (Dectin-1) is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily, predominantly expressed on myeloid cells. It is a type II membrane glycoprotein receptor with an extracellular C-type lectin-like domain and a cytoplasmic ITAM motif.
CLEC7A functions as a pattern-recognition receptor that detects β-1,3 and β-1,6-linked glucans from fungi and plants, contributing to innate immune responses. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, leading to reactive oxygen species formation and oxidative burst.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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