Thermo Fisher Scientific MID1 Polyclonal Antibody
Thermo Fisher Scientific의 MID1 Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 MID1 단백질을 검출하는 토끼 유래 IgG 항체입니다. Western blot, IHC, IP에 사용 가능하며, 고순도(>95%) 항원 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 71–120 of human Midline-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553463 |
Product Specific Information
- Detects endogenous protein at approximately 75 kDa.
- Purity >95% by SDS-PAGE.
Target Information
Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1.
It belongs to the TRIM/RBCC family and contains:
- Two B box-type zinc fingers
- One B30.2/SPRY domain
- One COS domain
- One fibronectin type-III domain
- One RING-type zinc finger
Midline-1 functions as an E3 ubiquitin ligase, targeting the catalytic subunit of protein phosphatase 2 for degradation.
It is a cytoplasmic protein forming homodimers or heterodimers with Midline-2 and interacts with IGBP1 (Lymphocyte signaling protein A4).
Defects in MID1 cause Opitz syndrome type I (OS-I), an X-linked recessive disorder characterized by:
- Hypertelorism
- Genital-urinary defects (hypospadias in males, splayed labia in females)
- Lip-palate-laryngotracheal clefts
- Imperforate anus
- Developmental delay
- Congenital heart defects
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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