Thermo Fisher Scientific NFYC Polyclonal Antibody
Thermo Fisher Scientific NFYC Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 NF-YC 단백질을 검출하는 고순도 항체입니다. Western blot 및 IHC(P) 실험에 적합하며, 항원 친화 크로마토그래피로 정제된 액상 제품으로 안정적인 신호를 제공합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 50–106 of human NF-YC |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553462 |
Product Specific Information
This antibody detects endogenous NF-YC protein at a molecular weight of 50 kDa.
Purity is >95% by SDS-PAGE.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y consists of three subunits: NF-YA, NF-YB, and NF-YC. All three are essential for DNA binding. Each NF-Y subunit contains a conserved segment necessary for NF-Y–DNA complex formation, homologous to the histone-fold motif found in eukaryotic histones. The DNA binding domains of NF-YB and NF-YC interact through a histone-fold “handshake” motif similar to the interaction between histone proteins H2B and H2A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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