Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (1400.24.17)
IL-1 beta 단백질을 표적하는 mouse monoclonal antibody로 WB, ELISA 등 다양한 응용에 적합. Recombinant mouse IL-1 beta를 면역원으로 사용하며, 17 kDa 단백질 검출 가능. Endotoxin ≤10 EU/mg, 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| ELISA | Assay-dependent | View 9 publications |
| Neutralization (Neu) | - | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1400.24.17 |
| Immunogen | Recombinant mouse IL-1 beta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223529 |
Additional Formats
Product Specific Information
MM425B targets IL-1 beta in WB and ELISA applications and shows reactivity with mouse samples.
The immunogen is recombinant mouse IL-1 beta.
MM425B detects IL-1 beta with a predicted molecular weight of approximately 17 kDa.
This antibody has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains ≤10 endotoxin units per milligram (EU/mg).
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.
It is synthesized as a 31 kDa inactive pro-form that accumulates in the cytosol and is cleaved into an active 17 kDa form via inflammasome activation.
IL-1 beta is secreted through a non-classical pathway alongside caspase-1.
It signals through IL-1RI and IL-1RII receptors, shared with IL-1 alpha, and its activity is regulated by IL-1 Receptor Antagonist (IL-1RA).
IL-1 beta plays a key role in innate host defense and is associated with chronic inflammatory conditions, making neutralizing antibodies potentially valuable for therapeutic research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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