Thermo Fisher Scientific IL-4 Monoclonal Antibody (1D11)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 26 publications 26 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1D11

Immunogen

Recombinant mouse IL-4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223530

Product Specific Information

MM450C targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples. Does not bind to human IL-4.

The MM450C immunogen is recombinant mouse IL-4.

MM450C detects IL-4 which has a predicted molecular weight of approximately 14 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

The MM450C mouse IL4 antibody (clone 1D11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM450DB (biotinylated conjugate of clone 24G2). Typical dilutions for sandwich ELISA include 1-5 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM450C (clone 1D11) and biotinylated antibody MM450DB (clone 24G2) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.