Thermo Fisher Scientific Human SIK1, GST Tag Recombinant Protein

Thermo Fisher Scientific Human SIK1, GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: -

Product Specifications

Product Specific Information

For maximum recovery, spin prior to use.
Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted.
For larger sizes, reduce freeze/thaws by preparing aliquots (not below 20 µL).
Never store kinase diluted.
Properly stored at -80°C, product guaranteed for 6 months from purchase.

This recombinant human SIK1 (1–303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance

Related Links:

• LanthaScreen™ Kinase Activity Assay
• LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant, Active, Catalytic
Gene Accession Number: NP_775490.2

Target Information

SIK1 may mediate the physiological effects of LKB1, including tumor suppressor function.
It is involved in signaling with proteins such as STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1–4, and MELK related to AMPK.
Phosphorylation at Thr322 activates SIK1, increasing catalytic activity of sodium/potassium ATPase α-subunit and intracellular sodium levels.
The kinase family is classified into 8 major groups based on catalytic domain sequence comparison (PTK/STK).
The STE group includes 50 kinases related to the MAPK cascade (Ste7/MAP2K, Ste11/MAP3K, Ste20/MAP4K).
MAP kinase cascades are well characterized in yeast pheromone response pathways, where pheromones bind to Ste cell surface receptors and activate MAPK signaling.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.