Thermo Fisher Scientific IL-6 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-6 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-6 (500-P56) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-6.

Western Blot:
To detect Murine IL-6 by Western Blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, detection limit for Recombinant Murine IL-6 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P56BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation. This endogenous pyrogen induces fever and is expressed at sites of acute and chronic inflammation. It triggers transcriptional inflammatory responses via the interleukin 6 receptor alpha. IL-6 function is linked to various disease states including diabetes mellitus susceptibility, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and rheumatoid arthritis. Elevated IL-6 levels are observed in viral infections, including COVID-19.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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