Thermo Fisher Scientific NFkB p65 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to amino acids within 1 and 50 of human p65 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFkB p65,uniProtId:Q04206-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:551,antigenImageFileName:PA1-41573_NFkB_p65_Q04206-1_Rabbit.svg,antigenImageFileNamePDP:PA1-41573_NFkB_p65_Q04206-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.2% gelatin

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1077255

Product Specific Information

Suggested positive control: antigen standard for RELA (transient overexpression lysate), Hela whole cell extract, HeLa cell lysate.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.