Thermo Fisher Scientific Nanog Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is expressed in pluripotent mouse and human cell lines but absent in differentiated cells.
Human NANOG protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. The human NANOG gene maps to locus 12p13.31, while the mouse gene maps to 6 F2.

Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research indicates that high levels of human NANOG are expressed in the undifferentiated N-Tera embryonal carcinoma cell line. NANOG functions as a transcription regulator involved in embryonic stem (ES) cell proliferation and self-renewal.

The role of NANOG in embryonic development suggests its utility in creating stem cells for cell replacement therapies in degenerative diseases. Artificial stem cells, or induced pluripotent stem (iPS) cells, can be generated by expressing NANOG along with transcription factors such as Sox2, Klf4, Lin28, and c-Myc, eliminating viral introduction and improving safety for regenerative medicine. Overexpression of NANOG promotes S-phase entry and proliferation. Dysfunctions in NANOG are associated with teratocarcinoma and germ cell/embryonal cancers.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.