Thermo Fisher Scientific PDCD1 Monoclonal Antibody (UMAB222), UltraMAB
PDCD1 단백질을 표적하는 UMAB222 단일클론 항체로 인간 및 마우스 시료에 반응. Western blot, Flow cytometry, ChIP, Peptide array 등 다양한 응용 가능. 재구성 및 결합 실험에 적합한 무보존제, 동결건조 형태. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB222 |
| Immunogen | Full length human recombinant protein of human PDCD1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
T-cell activation is regulated by PD-1 (also known as PDCD-1), which mediates inhibitory signals upon binding its ligands PDL-1 and PDL-2.
These interactions help prevent overactivation of immune responses in the absence of danger signals such as LPS.
PD-1 deficiency leads to hyperactivated immune systems and autoimmune conditions.
Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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