Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (C7), Functional Grade, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (C7), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The C7 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on NK cell lineages in both human and mouse. Mouse NKG2D binds to RAE1 variants (α, β, γ, δ, ε) and H60, costimulating NK activation receptors via DAP12/DAP10 adaptors.

C7 detects NK cells from all tested mouse strains and interferes with NKG2D-ligand interactions, inhibiting lysis of Ba/F3-RAE1d cells by C57BL/6 LAK cells. C7 competes with clone A10 for binding, suggesting similar epitopes or steric hindrance. Functionally, C7 acts as neutralizing, while A10 acts as activating.

Expression of NKG2D on mouse peripheral NK and NKT cells can be detected by flow cytometry using mAb CX5 with higher intensity.

Applications Reported: Flow cytometric analysis and in vitro blocking of mouse NKG2D.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation. They regulate humoral and cell-mediated immunity and express calcium-dependent C-type lectins involved in NK cell function. The NKG2 gene family encodes type II transmembrane proteins with C-type lectin domains, located within the NK complex region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.