Thermo Fisher Scientific BCL6 Monoclonal Antibody (BCL-DWN), APC, eBioscience
마우스 Bcl-6 단백질을 인식하는 단클론 항체로, APC 형광체가 결합되어 있습니다. 세포 내 염색 및 유세포분석(Flow Cytometry)에 적합하며, 0.03 µg/test 농도로 사용 가능합니다. 4°C 어두운 곳에서 보관하며 동결 금지. 연구용으로만 사용됩니다.
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Applications
- Flow Cytometry (Flow): Tested at 0.03 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BCL-DWN |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573213 |
Additional Formats
Product Specific Information
Description:
This BCL-DWN monoclonal antibody reacts with mouse Bcl-6 (B-cell lymphoma 6), a member of the POZ family of transcriptional repressors. Bcl-6 is abundantly expressed in germinal center B cells and in differentiating/activated B cells. The 79-kDa protein is essential for germinal center B cell proliferation and survival, and supports pre-B cell survival during development.
Bcl-6 is constitutively expressed in diffuse large B cell lymphomas (DLBCLs), Burkitt’s lymphoma, and non-Hodgkin’s lymphoma, and has been reported in CD4+ T follicular helper and memory T cells. It is antagonized by Blimp-1.
Applications Reported:
Reported for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of splenocytes from sheep red blood cell-immunized mice using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Refer to BestProtocols®: Protocol B for intracellular nuclear proteins.
Use ≤ 0.03 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined). Antibody titration recommended for optimal results.
Fluorescence Characteristics:
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Bcl-6 proto-oncogene product is a Kruppel-type zinc-finger protein mainly expressed in germinal center B cells and related lymphomas. It is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, with rearrangements detected in 33–45% of diffuse large B cell lymphomas. Bcl-6 is also detected in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas, and nodular lymphocyte-predominant Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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