Thermo Fisher Scientific NKIRAS1 Monoclonal Antibody (OTI1D2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

KappaB ras-1 (kappa-B-ras-1) and kappaB-ras-2 are small Ras-like GTPases that associate with Ikappa-B, an inhibitor of NF-kappa-B.
Ikappa-B exists in two homologous forms, Ikappa-B-alpha and Ikappa-B-beta, with Ikappa-B-beta containing a unique 47-amino acid region within its ankyrin domain.
Inactive Ikappa-B-alpha–NF-kappa-B complexes shuttle between nucleus and cytoplasm, while Ikappa-B-beta–NF-kappa-B complexes remain in the cytoplasm.
KappaB-ras proteins preferentially bind Ikappa-B-beta through this unique insert, modulating its localization and degradation rate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.