Thermo Fisher Scientific SERPINA12 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, Jurkat, Raji
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structural analysis of vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive features of Serpin family members. The serpins are irreversible (“suicidal”) serine-protease inhibitors, characterized by more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure containing an exposed reactive center loop acting as a pseudo-substrate for the target proteinase.

Members of this family are involved in fundamental biological processes including:

• Blood coagulation
• Fibrinolysis
• Complement activation
• Angiogenesis
• Inflammation
• Tumor suppression

In humans, serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin, though its protease inhibitory activity remains unknown. Vaspin mRNA expression in visceral fat correlates positively with BMI and body fat percentage. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, normalizing blood glucose levels and reversing altered expression of diabetes-relevant adipocytokines such as leptin, adiponectin, resistin, and TNF-alpha. These findings suggest potential clinical use of Vaspin in ameliorating metabolic syndrome-related abnormalities.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.