Thermo Fisher Scientific H3K36me3 Recombinant Rabbit Monoclonal Antibody (RM155), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

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Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

0.2-1 µg/mL

-

ChIP assay (ChIP)

1-5 µg

-

ChIP-sequencing (ChIP-Seq)

3 µg

-

Luminex (LUM)

0.1-0.5 µg/mL

-

Peptide Array (Array)

0.25 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM155

Immunogen

Tri-methyl-peptide corresponding to tri-methyl-Histone H3 (Lys36).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 50% glycerol, 1% BSA

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2661912

Product Specific Information

This antibody reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in histone H3.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

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Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.