Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-10. Anti-Rat IL-10-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IL-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-10 (500-P139) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-10.

Western Blot:
To detect Rat IL-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-10 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P139BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes.
This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages.
It enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway.
Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract.
Mutations in this gene have been associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.