Thermo Fisher Scientific Amphiregulin Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic 19-mer peptide, corresponding to aa 8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Amphiregulin,uniProtId:P15514-1,ncbiNodeId:9606,antigenRange:8-26,antigenLength:252,antigenImageFileName:PA5-16616_Amphiregulin_P15514-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16616_Amphiregulin_P15514-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984210

Product Specific Information

PA5-16616 targets Amphiregulin in IP applications and shows reactivity with Human and mouse samples.

The PA5-16616 immunogen is a synthetic 19-mer peptide, corresponding to aa 8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin.

Target Information

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.