Thermo Fisher Scientific Kappa Light Chain Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

kappa light chains purified from human Bence Jones proteins if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kappa Light Chain,uniProtId:P01834-1,ncbiNodeId:9606,antigenRange:1-107,antigenLength:107,antigenImageFileName:PA5-16647_Kappa_Light_Chain_P01834-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16647_Kappa_Light_Chain_P01834-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Kappa Light Chain,uniProtId:P01602-1,ncbiNodeId:9606,antigenRange:1-117,antigenLength:117,antigenImageFileName:PA5-16647_Kappa_Light_Chain_P01602-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16647_Kappa_Light_Chain_P01602-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Kappa Light Chain,uniProtId:P0DOX7-1,ncbiNodeId:9606,antigenRange:1-214,antigenLength:214,antigenImageFileName:PA5-16647_Kappa_Light_Chain_P0DOX7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16647_Kappa_Light_Chain_P0DOX7-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982326

Product Specific Information

PA5-16647 targets Kappa Light Chain in IHC (P) applications and shows reactivity with Human, baboon, monkey and rat samples. Does not react with dog samples. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

The PA5-16647 immunogen is kappa light chains purified from human Bence Jones proteins.

Target Information

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin`s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.