Thermo Fisher Scientific UPF1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 819-1118 of human UPF1/RENT1 (NP_0029022) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UPF1,uniProtId:Q92900-1,ncbiNodeId:9606,antigenRange:819-1118,antigenLength:1129,antigenImageFileName:PA5-90296_UPF1_Q92900-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90296_UPF1_Q92900-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806006

Product Specific Information

Positive Samples: 293T, SH-SY5Y, K-562, HL-60, Mouse liver; Cellular Location: Cytoplasm, Nucleus, P-body

Immunogen sequence: QEVEIASVDA FQGREKDFII LSCVRANEHQ GIGFLNDPRR LNVALTRARY GVIIVGNPKA LSKQPLWNHL LNYYKEQKVL VEGPLNNLRE SLMQFSKPRK LVNTINPGAR FMTTAMYDAR EAIIPGSVYD RSSQGRPSSM YFQTHDQIGM ISAGPSHVAA MNIPIPFNLV MPPMPPPGYF GQANGPAAGR GTPKGKTGRG GRQKNRFGLP GPSQTNLPNS QASQDVASQP FSQGALTQGY ISMSQPSQMS QPGLSQPELS QDSYLGDEFK SQIDVALSQD STYQGERAYQ HGGVTGLSQY

Target Information

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.