
Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience
PE 형광으로 표지된 Mouse IgG2a kappa 동형 대조 항체로, 유세포 분석 시 비특이적 결합 확인용으로 사용. 488–561 nm 여기 및 578 nm 방출 특성. 친화 크로마토그래피로 정제되어 안정적이며, 4°C 암소 보관 권장. 연구용 전용 시약.
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Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBM2a |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1603328 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805 nm)
- Brilliant Violet series (421–786 nm)
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780 nm)
- Custom conjugation available
Product Specific Information
This monoclonal mouse IgG2a, kappa antibody serves as an isotype control for mouse IgG2a antibodies.
Applications Reported: Suitable for cell surface and intracellular flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in a final volume of 100 µL. Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm; Emission: 578 nm; Compatible lasers: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a key role in the adaptive immune response, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It regulates immune processes such as T cell activation and B cell inhibition.
Widely used in research applications like ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic studies including immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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