
Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience
Rat IgG2a kappa 동형 대조군으로, PE 형광표지 항체입니다. Flow cytometry 및 ICC/IF에 사용되며, 비특이적 결합 평가용으로 적합합니다. 0.2 mg/mL 농도의 액상 제품으로, 4°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834380 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
- Description: Monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
- Applications Reported: Reported for use in flow cytometric analysis.
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use at the same concentration as the experimental antibody.
- Excitation/Emission: 488–561 nm / 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Rat IgG2a is an immunoglobulin subclass produced by the rat immune system, known for complement activation and mediating ADCC.
It has a molecular weight of approximately 160 kDa, consisting of two heavy and two light chains.
Commonly used as a control or reference antibody in experiments involving rat models, and in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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