Thermo Fisher Scientific F(ab`)2-Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PE, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

1 µg/test

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

PE PE PE

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 594/host/Donkey/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Biotin/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/HRP/host/Donkey/)
• [Qdot 525](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 525/host/Donkey/)
• [Qdot 565](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 565/host/Donkey/)
• [Qdot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 625/host/Donkey/)
• [Qdot 655](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 655/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210761

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: The anti-rabbit IgG polyclonal antibody reacts with the heavy and light chains of rabbit IgG, with minimal cross reaction to bovine, guinea pig, Syrian hamster, horse, human, mouse, rat and sheep serum proteins. Reactions to non-immunoglobulin rabbit serum proteins have not been detected.

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.

Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis to detect polyclonal rabbit antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.