Thermo Fisher Scientific Mouse IgG3 Isotype Control (B10), PE, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

10 µL/test

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG3, lambda

Class

Monoclonal

Type

Isotype Control

Clone

B10

Conjugate

PE PE PE

View additional formats

• Unconjugated
• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

10 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10733011

Product Specific Information

Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.

Applications Reported: This mouse IgG3 isotype control has been reported for use in flow cytometric analysis.

Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 10 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. .

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.