Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PerCP-eFluor 710, eBioscience
FOXP3 단백질을 인식하는 FJK-16s 클론 단일클론 항체로, PerCP-eFluor 710 형광체로 결합되어 Flow Cytometry용으로 최적화됨. 마우스, 랫, 개, 돼지, 소, 고양이에 반응하며 Treg 세포 연구에 적합. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 17 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 92 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811810 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, Biotin
- Brilliant Violet 421, 605, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation available
Product Specific Information
The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2).
Foxp3 is a 49–55 kDa transcription factor essential for regulatory T cell (Treg) function.
Immunoblotting mapped the epitope to amino acids 75–125 of mouse Foxp3.
Optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00).
Use of other fixation and staining buffers is not recommended.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Recommended use ≤0.25 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorophore Information:
PerCP-eFluor 710 emits at 710 nm, excited by 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Sensitive to photo-induced oxidation—protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness.
Excitation: 488 nm
Emission: 710 nm
Laser: Blue
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis.
It is highly expressed in CD25⁺CD4⁺ regulatory T cells, low in CD4⁺CD25⁻ cells, and absent in CD4⁻CD8⁺ T cells.
FOXP3 defects cause scurfy phenotype in mice and IPEX syndrome in humans.
Transcript variants encoding different isoforms have been identified.
In human cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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