Thermo Fisher Scientific TNNI3 Monoclonal Antibody (OTI8G8), TrueMAB
Cardiac Troponin I 단백질을 인식하는 Mouse monoclonal antibody로, Western blot 및 IHC(P) 검증 완료. E.coli 발현 재조합 단백질(aa 1–210)을 면역원으로 사용. 동결건조 형태로 제공되며, PBS buffer(8% trehalose) 내 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8G8 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 1–210 of human TNNI3 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cardiac Troponin I (TNNI3) is a cardiac-specific inhibitory protein that forms part of the troponin complex with troponin C (TnC) and troponin T (TnT).
It regulates muscle contraction by modulating actin–myosin interactions in response to calcium binding.
Measurement of cardiac TnI and TnT levels is a key diagnostic indicator of myocardial injury, aiding differentiation between angina and myocardial infarction.
Mutations in the TNNI3 gene are associated with familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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