Thermo Fisher Scientific Phospho-TRIM28 (Ser824) Recombinant Rabbit Monoclonal Antibody (6H11L46)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Mouse, and Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

Target Information

The KRAB (Kruppel-associated box) domain, approximately 45 amino acids in length, is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome.

These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing RING finger, B boxes, and PHD finger domains. KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 targets KRAZ1 to centromeric heterochromatin regions containing HP1alpha, thereby regulating transcriptional repression. Mutant KAP-1 unable to bind HP1 leads to random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.