Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), PE-Cyanine7, eBioscience
인간 GARP 단백질을 인식하는 G14D9 단클론 항체로, PE-Cyanine7 형광체가 결합된 제품입니다. Flow Cytometry 분석용으로 최적화되어 있으며, 4°C 암소 보관 및 광민감성 주의가 필요합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), PE-Cyanine7, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (1 µg)/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G14D9 |
| Immunogen | A mixture of mGARP-Fc and hGARP 21H-F1 & -F2 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2573559 |
Additional Formats
Product Specific Information
Description:
The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an ~80 kDa glycoprotein expressed on the cell surface. Expression has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression is observed on megakaryocytes, platelets, and activated regulatory T (Treg) cells. GARP expression on activated Treg cells is necessary for their suppressive function, possibly as a receptor for LAP/TGF beta.
Applications Reported:
This G14D9 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended usage: 5 µL (1 µg) per test (100 µL final volume). Optimal cell number: 10⁵–10⁸ cells/test.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
Excitation / Emission:
488–561 nm / 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
GARP (leucine rich repeat containing 32) is a type I membrane protein with 22 leucine-rich repeats and a short intracytoplasmic region. It is expressed in CD4+, CD25+, FoxP3+ cells (Tregs) and may serve as a surface marker for Treg cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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