Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-Cyanine7, eBioscience

Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (intracellular staining)

Tested Dilution: ≤ 0.25 µg/test
Publications: View 18 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.

• In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation via interaction with PU.1 and transcription of B cell–specific genes such as Prdm1 (Blimp1).
• In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 cell development and induces Blimp1 and IL-10 expression.
• IRF4 is also involved in macrophage polarization and regulation.
• Overexpression of IRF4 has been observed in various hematologic malignancies.

Applications Reported:
For intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested using intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells with the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
Recommended use: ≤ 0.25 µg per test (100 µL final volume; 10⁵–10⁸ cells/test).
Antibody should be titrated for optimal performance.

Light Sensitivity:
PE-Cyanine7 is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-822-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The IRF (interferon regulatory factor) family of transcription factors features a unique tryptophan pentad repeat DNA-binding domain. IRFs regulate interferon responses to viral infection and control interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates TLR signaling, a key pathway in innate and adaptive immunity.
A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH may contribute to multiple myeloma.
Alternative splicing variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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