Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-Cyanine7, eBioscience

Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (intracellular staining)

Tested Dilution: ≤ 0.25 µg/test
Publications: View 18 publications

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 700
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

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Product Specific Information

Description:
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.

• In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation via interaction with PU.1 and transcription of B cell–specific genes such as Prdm1 (Blimp1).
• In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 cell development and induces Blimp1 and IL-10 expression.
• IRF4 is also involved in macrophage polarization and regulation.
• Overexpression of IRF4 has been observed in various hematologic malignancies.

Applications Reported:
For intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested using intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells with the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
Recommended use: ≤ 0.25 µg per test (100 µL final volume; 10⁵–10⁸ cells/test).
Antibody should be titrated for optimal performance.

Light Sensitivity:
PE-Cyanine7 is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-822-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

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Target Information

The IRF (interferon regulatory factor) family of transcription factors features a unique tryptophan pentad repeat DNA-binding domain. IRFs regulate interferon responses to viral infection and control interferon-inducible genes.
IRF4 is lymphocyte-specific and negatively regulates TLR signaling, a key pathway in innate and adaptive immunity.
A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH may contribute to multiple myeloma.
Alternative splicing variants have been identified.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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