Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Yellow 755, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells (B-1 cells). CD5 acts as a counter-receptor for CD72 and is involved in T-B cell interactions.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported:
For use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Use ≤ 0.2 µg per test in 100 µL final volume. Adjust cell number empirically (10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis with NovaFluor dyes.
• Include CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: add 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures that provide engineered fluorescent signatures with minimized spillover and spread.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow Laser)

Note:
Internal testing shows NovaFluor Yellow 755 may non-specifically stain B cells in SJL mice. No such staining observed in BALB/c or C57BL/6 strains.

Target Information

CD5 is a 67 kDa single-chain transmembrane glycoprotein found on most human T-lymphocytes, thymocytes, and certain B-cell subsets. It plays a regulatory role in T-cell activation and proliferation. CD5 expression is associated with various T-cell leukemias and lymphomas.
Diseases linked to CD5 dysfunction include thymus cancer and Richter’s Syndrome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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