Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), NovaFluor™ Blue 510

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, eFluor 506
• FITC
• NovaFluor Blue 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) expressed in CD45.2 mouse strains such as C57BL/6, BALB/c, C58, DBA, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample or per 100 µL cell suspension.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures with minimized spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins.
It regulates Src-family kinases, plays a role in lymphocyte development, antigen signaling, integrin-mediated survival pathways, and apoptosis-related DNA fragmentation.
CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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