Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), NovaFluor Blue 555, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, eFluor 506
• FITC
• NovaFluor Blue 510, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Custom conjugation available upon request

Product Specific Information

Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
The 104 antibody is suitable for flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤0.5 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Titrate antibody for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample (regardless of NovaFluor count) before mixing with cells.
  • For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spectral spillover and spread.

Optical Properties:
Excitation: 496 nm
Emission: 555 nm
Laser: 488 nm (Blue)

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, lymphocyte development, and antigen signaling. Multiple isoforms arise via alternative splicing, differing in extracellular domains and membrane localization. CD45.2 also modulates integrin-mediated signaling and participates in apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted lymphoid expression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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