Thermo Fisher Scientific Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody (SPM180)
Factor XIIIa 단일클론 항체(SPM180)는 인간 반응성을 가지며 다양한 면역실험(WB, IHC, ICC, Flow, ELISA)에 사용 가능. Protein A/G로 정제된 액상 형태로 제공되며, 4°C 보관. 혈액 응고 인자 XIIIa 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | 2–4 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM180 |
| Immunogen | Recombinant fragment of human Factor XIIIa protein (aa46–181) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of two A subunits and two B subunits. The A subunits have catalytic function, while the B subunits serve as plasma carrier molecules. Platelet factor XIII consists only of two A subunits, identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ions, plasma factor XIII dissociates its B subunits and yields the active enzyme, factor XIIIa. This enzyme acts as a transglutaminase to catalyze gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor or fibronectin to fibrin alpha chains. Factor XIII deficiency can cause bleeding tendency, defective wound healing, and habitual abortion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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