Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, PeproTech
마우스 IL-12 p70에 특이적인 염소 유래 폴리클로날 항체로, Western Blot과 ELISA 등 다양한 면역 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 무보존 PBS 용액으로 제공됩니다. 연구용으로 면역 반응 및 사이토카인 분석에 활용됩니다.
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Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| Neutralization (Neu) | – | View 2 publications |
| In vitro Assay (IV) | – | View 5 publications |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Murine IL-12 p70 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929522 |
Product Specific Information
AA Sequence of Recombinant Protein
- p35 subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA
- p40 subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-12 matrix.
Sandwich ELISA:
To detect Murine IL-12 by sandwich ELISA (using 100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. In conjunction with PeproTech Biotinylated Anti-Murine IL-12 (500-P155GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-12.
Western Blot:
For Western Blot analysis, use 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant Murine IL-12 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-12 p70 (interleukin-12) is a cytokine critical for immune responses to infections and cancer. Produced by dendritic cells, macrophages, and B cells, it stimulates interferon-gamma production and activates T cells and NK cells. IL-12 p70 is studied as a therapeutic target for infectious diseases, autoimmune disorders, and cancer, enhancing immune responses and modulating inflammation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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