Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech
인간 IL-12 p70를 특이적으로 인식하는 Biotin 결합 염소 폴리클로날 항체. Western blot 및 ELISA에 최적화되어 있으며, 고순도 재조합 단백질로부터 제작. 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공.
- 카탈로그번호
- 500-P154HGBT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEK293 cells-derived Recombinant Human IL-12 p70 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2942213 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein
- p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
- p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Preparation
Produced from sera of goats immunized with highly pure Recombinant Human IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA
To detect Human IL-12 by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Human IL-12 (500-P154HG) as a capture antibody, it allows detection of at least 2000–4000 pg/mL of Recombinant Human IL-12.
Western Blot
To detect Human IL-12 by Western Blot, use 0.1–0.2 µg/mL. With compatible development reagents, the detection limit for Recombinant Human IL-12 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging
500-P154HGBT-1MG is provided as 2 × 500 µg.
Target Information
IL-12 p70 (interleukin-12) is a cytokine critical for immune responses against infections and cancer. It is produced by dendritic cells, macrophages, and B cells in response to pathogens and tumor cells. IL-12 p70 stimulates interferon-gamma production, promoting T cell and NK cell activation. It is studied as a therapeutic target for infectious diseases, autoimmune disorders, and cancer due to its role in enhancing immune responses and modulating inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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