Thermo Fisher Scientific HSP27 Monoclonal Antibody (8A7), FITC
HSP27 단백질을 검출하기 위한 FITC 결합 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 여러 종에 반응하며, 높은 특이성과 재현성을 제공합니다. 단백질 G로 정제된 액상 형태로 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:5,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:5,000 | |
| Immunocytochemistry (ICC/IF) | 1:200 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Sheep, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8A7 |
| Immunogen | HSP27 peptide |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931576 |
Additional Formats
- APC (Catalog No. MA5-45121)
- PerCP (Catalog No. MA5-45123)
- Custom conjugation available upon request
Product Specific Information
A 1:5000 dilution of MA5-45122 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis. Detects approximately 25 kDa or 27 kDa. Recognizes HSP25 and HSP27, and cross-reacts with alpha B crystallin.
Target Information
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization, and regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins. Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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