Thermo Fisher Scientific HSP90 Monoclonal Antibody (AC-16), APC
HSP90 단백질 검출용 APC 결합 마우스 단클론 항체. Western blot, IHC, Microarray 등에 사용 가능. 인간, 마우스, 식물 등 다양한 종에 반응. 단백질 접힘 및 스트레스 반응 연구에 적합. 4°C 보관, Protein G 정제.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Fungi, Human, Insect, Mouse, Plant, Rabbit, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC-16 |
| Immunogen | Heat shock protein 90 from the water mold Achyla ambisexualis |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931569 |
Additional Formats
- FITC (Cat. No. MA5-45116)
- PerCP (Cat. No. MA5-45117)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45115 was sufficient for detection of HSP90 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects 90 kDa.
This antibody is reactive with both the constitutive and inducible forms of HSP90. It does not bind to the native form and does not recognize HSP90 from E. coli or yeast.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones to fold newly synthesized proteins or refold denatured proteins after stress.
Two major cytosolic forms exist: HSP90AA1 (inducible) and HSP90AB1 (constitutive), as well as mitochondrial variants (Chen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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