Thermo Fisher Scientific HSP27 Monoclonal Antibody (8A7), APC
HSP27 단백질 검출용 APC 결합 단일클론 항체로 다양한 종에서 반응하며 WB, IHC, ICC, Flow 등 다중 응용 가능. 높은 특이성과 감도로 HSP25/HSP27 및 αB crystallin 교차 반응. 연구용으로 세포 스트레스 및 단백질 접힘 연구에 적합.
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Thermo Fisher Scientific HSP27 Monoclonal Antibody (8A7), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Sheep, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8A7 |
| Immunogen | HSP27 peptide |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931575 |
Available Formats
- FITC conjugate: MA5-45122
- PerCP conjugate: MA5-45123
- Custom conjugation available upon request
Product Specific Information
A 1:5000 dilution of MA5-45121 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Detects approximately 25 kDa or 27 kDa. Recognizes HSP25 and HSP27, cross-reacts with alpha B crystallin.
Target Information
In response to environmental stress, cells increase expression of heat shock or stress proteins.
HSPB1 (heat shock protein beta-1 or HSP27) is a small molecular chaperone that maintains denatured proteins in a folding-competent state.
It contributes to stress resistance and actin organization, and regulates phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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