Thermo Fisher Scientific HSP90 Monoclonal Antibody (AC-16), FITC
HSP90 단백질 검출용 FITC 결합 단일클론 항체로, WB, IHC 등 다양한 응용에 적합. Mouse IgG2b 형, Protein G 정제, 4°C 암소 보관. Heat shock protein 90 인식, E.coli 및 yeast에는 반응하지 않음.
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Thermo Fisher Scientific HSP90 Monoclonal Antibody (AC-16), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Fungi, Human, Insect, Mouse, Plant, Rabbit, Rat |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC-16 |
| Immunogen | Heat shock protein 90 from the water mold Achyla ambisexualis |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931570 |
Additional Formats
- APC: MA5-45115
- PerCP: MA5-45117
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45116 was sufficient for detection of HSP90 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects 90 kDa. This antibody is reactive with both the constitutive and inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E. coli or yeast.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones to fold newly synthesized proteins or refold denatured proteins after stress.
There are two major cytosolic forms: HSP90AA1 (inducible) and HSP90AB1, as well as mitochondrial variants (Chen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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