Thermo Fisher Scientific Sp1 Polyclonal Antibody
Sp1 전사인자에 특이적인 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 인간과 마우스 반응성, 항원 친화 크로마토그래피로 정제, 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 735 to 785 of Human Sp1 Transcription Factor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses. SP1 is highly regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate the cellular response to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter, as well as in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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