Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience
Flow cytometry용 Rat IgG2a kappa 아이소타입 대조항체로, eBR2a 클론 및 PE-eFluor 610 형광체 결합. 488–561 nm에서 여기, 607 nm에서 방출. 광산화에 민감하며 4°C 암소 보관 권장. 실험 항체와 동일 농도로 사용 권장.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637340 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- FITC
- Functional Grade
- Pacific Orange
- PE
- PE-Cyanine5 / 5.5 / 7
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available on request
Product Specific Information
The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.
This eBR2a antibody is reported for use in flow cytometric analysis.
It has been tested by flow cytometric analysis of mouse splenocytes.
Use the isotype control at the same concentration as the experimental antibody.
Fluorophore characteristics:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
Ensure instrument compatibility before use.
Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.
Excitation / Emission / Laser:
488–561 nm excitation; 607 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2a is an immunoglobulin subclass produced by the rat immune system.
It activates complement and mediates antibody-dependent cell-mediated cytotoxicity (ADCC).
Molecular weight: approximately 160 kDa (two heavy chains, two light chains).
Commonly used as a control or reference antibody in rat model experiments, flow cytometry, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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