Thermo Fisher Scientific Rat IgM Isotype Control (eBRM), PE-eFluor 610, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBRM

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

View additional formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine5.5
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637351

Product Specific Information

Description: The monoclonal rat IgM K is useful as an isotype control immunoglobulin.

Applications Reported: Rat IgM Isotype Control PE-eFluor® 610 has been reported for use in flow cytometric analysis.

Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses. Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology. It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders. Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.