Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), PE-eFluor 610, eBioscience
Flow cytometry용 Mouse IgG2b kappa 동형 대조군 항체로, PE-eFluor 610 형광체로 결합되어 있습니다. 488–561 nm 레이저로 여기되며 607 nm에서 방출됩니다. 실험 항체와 동일 농도로 사용하며, 광산화에 민감하므로 어둡게 보관하십시오.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637336 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 570, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Functional Grade
- Custom conjugation available
Product Specific Information
The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on normal human peripheral blood cells. Use at the same concentration as the experimental antibody.
- Fluorochrome Information: PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended detection with a 610/20 band pass filter (equivalent to PE-Texas Red®).
- Light Sensitivity: Sensitive to photo-induced oxidation. Protect from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.
- Excitation/Emission: 488–561 nm / 607 nm
- Laser Compatibility: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody produced by B cells in response to an antigen. It is the third most abundant IgG subtype in serum and plays a crucial role in adaptive immune response, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in immune regulation and is widely used in research and diagnostic applications such as ELISA, Western blotting, and immunohistochemistry, as well as in immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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